ACTIVITY AND SPECTROSCOPIC PROPERTIES OF BOVINE LIVER CATALASE IN SODIUM BIS(2-ETHYLHEXYL)SULFOSUCCINATE ISOOCTANE REVERSE MICELLES

Spectroscopic properties such as ultraviolet-visible spectroscopy, circular dichroism, steady state fluorescence and the catalatic activity of bovine liver catalase (BLC) have been studied in reverse-micelles microemulsions formed by sodium bis(2-ethylhexyl)sulfosuccinate (AOT) in isooctane. Activity measurements were carried out with the highly water soluble hydrogen peroxide as substrate as a function of temperature and pH, at various concentrations of substrate, enzyme, AOT and at different w0 values (w0 = [H2O]/[AOT]). The folding of catalase in reverse micelles is not drastically altered, although there are changes in the local surrounding of the prosthetic group. Kinetic measurements have been carried out under first-order conditions at low substrate concentration. They indicate that the measured substrate decomposition rate is limited by the exchange of substrate molecules between the substrate-filled and the enzyme-containing micelles. The specificity constant, k(s) = k(cat)/K(M), as related to water pool concentrations, k(s,wp)rm was, at all w0 values, considerably lower than k(s) measured in buffer (k(s)b). In contrast, overall values of k(s) in reverse micelles, k(s,ov)rm, were always 2-4-times higher than k(s)b. This apparent superactivity can, however, be ascribed to concentration effects. No 'bell-curve' to describe the w0 dependence of catalase activity in reverse micelles was found, k or k(s) increasing monotonously up to w0 = 50.