ASCORBIC ACID IS THE OXIDASE SUBSTRATE OF HORSERADISH PEROXIDASE

被引：0

作者：

Rogozhin, V. V. ^{[1
]}

Peretolchin, D. V. ^{[1
]}

Rogozhina, T. V. ^{[1
]}

机构：

[1] Yakutsk State Agr Acad, 15 Krasilnikova, Yakutsk 677007, Russia

来源：

IZVESTIYA VUZOV-PRIKLADNAYA KHIMIYA I BIOTEKHNOLOGIYA | 2013年 / 01期

关键词：

enzyme; steady-state kinetics; horseradish peroxidase; ascorbic acid; antioxidants; oxidation;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The steady-state kinetics of ascorbic acid oxidation catalyzed by horseradish peroxidase was studied. Ascorbic acid is the slowly oxidized substrate of peroxidase. The k(cat) and K-m values of this substrate were determined at pH from 3,5 to 8. At high concentrations (80-240 mu M), ascorbic acid activates the enzyme at all pH values. Activation mechanism by ascorbic acid of peroxidase oxidation reactions was suggested.

引用

页码：81 / +

页数：7

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