CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF NITRIC-OXIDE REDUCTASE CYTOCHROME P450NOR FROM FUSARIUM-OXYSPORUM

被引：31

作者：

NAKAHARA, K ^{[1
]}

SHOUN, H ^{[1
]}

ADACHI, S ^{[1
]}

IIZUKA, T ^{[1
]}

SHIRO, Y ^{[1
]}

机构：

[1] INST PHYS & CHEM RES,WAKO,SAITAMA 35101,JAPAN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 239卷 / 01期

关键词：

CYTOCHROME P450; NITRIC OXIDE REDUCTASE; FUSARIUM OXYSPORUM; CRYSTALLIZATION; X-RAY DIFFRACTION;

D O I：

10.1006/jmbi.1994.1355

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of cytochrome P450nor (P450nor), nitric oxide reductase from Fusarium oxysporum, were obtained by means of the hanging-drop vapour diffusion technique in the presence of 1.88 M ammonium sulphate (pH 7.2). They belong to the monoclinic space group P21, with unit cell dimensions of α = 74·7 Å, b = 86·7 Å, c = 62·0 Å and β = 97°. There are two molecules of P450nor in the asymmetric unit. The crystal diffracted to beyond 2.5 Å resolution and is suitable for X-ray crystallographic study. © 1994 Academic Press, Inc.

引用

页码：158 / 159

页数：2

共 11 条

[1] THE PROCESSING OF DIFFRACTION DATA TAKEN ON A SCREENLESS WEISSENBERG CAMERA FOR MACROMOLECULAR CRYSTALLOGRAPHY [J].