VACUOLAR PROTON-PUMPING PYROPHOSPHATASE IN BETA-VULGARIS SHOWS VECTORIAL ACTIVATION BY POTASSIUM

被引：38

作者：

DAVIES, JM

REA, PA

SANDERS, D

机构：

[1] UNIV YORK,DEPT BIOL,YORK YO1 5DD,N YORKSHIRE,ENGLAND

[2] UNIV PENN,DEPT BIOL,INST PLANT SCI,PHILADELPHIA,PA 19104

来源：

FEBS LETTERS | 1991年 / 278卷 / 01期

关键词：

H+-PYROPHOSPHATASE; POTASSIUM; TONOPLAST; PATCH CLAMP; BETA-VULGARIS;

D O I：

10.1016/0014-5793(91)80085-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous work with membrane vesicles has demonstrated an absolute dependence on K+ for proton translocation by the inorganic pyrophosphatase (H+ -PPase: EC 3.6.1.1) from the vacuolar membrane (tonoplast) of higher plants. Using intact vacuoles from sugar beet (Beta vulgaris) storage tissue, we have monitored PP(i)-dependent currents by patch clamp in 'whole vacuole' mode. Serial K+ substitutions were made at both tonoplast faces. The results show that K+ activation occurs only at the cytosolic face.

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页码：66 / 68

页数：3

相关论文

共 16 条

[1] SEPARATION OF TONOPLAST AND PLASMA-MEMBRANE POTENTIAL AND RESISTANCE IN CELLS OF OAT COLEOPTILES [J].

BATES, GW ;

GOLDSMITH, MHM ;

GOLDSMITH, TH .

JOURNAL OF MEMBRANE BIOLOGY, 1982, 66 (01) :15-23

[2]

BLACKFORD S, 1990, J BIOL CHEM, V265, P9617

[3] NA+/H+ ANTIPORT IN ISOLATED TONOPLAST VESICLES FROM STORAGE TISSUE OF BETA-VULGARIS [J].

BLUMWALD, E ;

POOLE, RJ .

PLANT PHYSIOLOGY, 1985, 78 (01) :163-167

[4] IDENTIFICATION AND PURIFICATION OF SUBSTRATE-BINDING SUBUNIT OF HIGHER-PLANT H+-TRANSLOCATING INORGANIC PYROPHOSPHATASE [J].

BRITTEN, CJ ;

TURNER, JC ;

REA, PA .

FEBS LETTERS, 1989, 256 (1-2) :200-206

[5] ION CHANNELS AND ATP-DRIVEN PUMPS INVOLVED IN ION-TRANSPORT ACROSS THE TONOPLAST OF SUGAR-BEET VACUOLES [J].

COYAUD, L ;

KURKDJIAN, A ;

KADO, R ;

HEDRICH, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 902 (02) :263-268

[6] NACI INDUCES A NA+/H+ ANTIPORT IN TONOPLAST VESICLES FROM BARLEY ROOTS [J].

GARBARINO, J ;

DUPONT, FM .

PLANT PHYSIOLOGY, 1988, 86 (01) :231-236

[7] COMPARATIVE STUDIES ON THE ELECTRICAL-PROPERTIES OF THE H+ TRANSLOCATING ATPASE AND PYROPHOSPHATASE OF THE VACUOLAR-LYSOSOMAL COMPARTMENT [J].

HEDRICH, R ;

KURKDJIAN, A ;

GUERN, J ;

FLUGGE, UI .

EMBO JOURNAL, 1989, 8 (10) :2835-2841

[8] CHARACTERIZATION OF AN ANION-PERMEABLE CHANNEL FROM SUGAR-BEET VACUOLES - EFFECT OF INHIBITORS [J].

HEDRICH, R ;

KURKDJIAN, A .

EMBO JOURNAL, 1988, 7 (12) :3661-3666

[9] PROTON GRADIENT ACROSS THE TONOPLAST OF RICCIA-FLUITANS AS A RESULT OF THE JOINT ACTION OF 2 ELECTROENZYMES [J].

JOHANNES, E ;

FELLE, H .

PLANT PHYSIOLOGY, 1990, 93 (02) :412-417

[10] A HYPOTHESIS RELATING CRITICAL POTASSIUM CONCENTRATIONS FOR GROWTH TO THE DISTRIBUTION AND FUNCTIONS OF THIS ION IN THE PLANT-CELL [J].

LEIGH, RA ;

JONES, RGW .

NEW PHYTOLOGIST, 1984, 97 (01) :1-13