2058 EXPRESSED SEQUENCE TAGS (ESTS) FROM A HUMAN FETAL LUNG CDNA LIBRARY

被引：43

作者：

SUDO, K

CHINEN, K

NAKAMURA, Y

机构：

[1] JAPANESE FDN CANC RES,INST CANC,DEPT BIOCHEM,TOSHIMA KU,TOKYO 170,JAPAN

[2] MITSUBISHI YUKA BCL CO LTD,TOKYO,TOKYO 174,JAPAN

来源：

GENOMICS | 1994年 / 24卷 / 02期

关键词：

D O I：

10.1006/geno.1994.1616

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

ESTs (expressed sequence tags) provide complementary resources for structural and functional analyses of the human genome. We have performed single-pass sequencing of 2058 randomly selected, directionally cloned cDNAs isolated from a fetal-lung cDNA library constructed with oligo(dT) primers, Computer analyses of the 5'-end sequences revealed that 60.4% of the clones were considered to be identical to previously reported human genes or ESTs; 9.0% of them showed significant homology to known genes in human, other mammals, or lower organisms; 30.6% showed no homology to any genes or DNA sequences in the public database. These data and reagents will be useful for future investigations of gene expression during prenatal development of human lung. (C) 1993 Academic Press, Inc.

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页码：276 / 279

页数：4

相关论文

共 11 条

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