HISTOCYTOLOGICAL STUDY OF APICES FROM INVITRO PLANTLETS OF DATE PALM (PHOENIX-DACTYLIFERA L) DURING A CRYOPRESERVATION PROCESS

被引：0
作者：
BAGNIOL, S
ENGELMANN, F
MICHAUXFERRIERE, N
机构：
[1] ORSTOM,RESSOURCES GENET & AMELIORAT PLANTES TROP LAB,911 AV AGROPOLIS,BP 5045,F-34032 MONTPELLIER,FRANCE
[2] GRFP,CONSERVATOIRE NATL BOT PORQUEROLLES,F-83400 ILE PORQUEROLLES,FRANCE
[3] CIRAD,BIOTROP,F-34032 MONTPELLIER,FRANCE
来源：
CRYO-LETTERS | 1992年 / 13卷 / 06期
关键词：
DATE PALM; PHOENIX-DACTYLIFERA; CRYOPRESERVATION; HISTOCYTOLOGY; APEX;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
An histo-cytological study was performed on apices of in vitro plantlets of date palm submitted to a cryopreservation process. A prerequisite 24-hour preculture on standard medium induced the recovery of cellular activity and the initiation of starch synthesis in some samples. This starch synthesis intensified during the pregrowth treatment. Cells which survived in liquid nitrogen showed characteristics of cells under osmotic stress. The first cell divisions were observed 4 days after thawing. In some samples, the cell multiplication first gave rise to a callus. Each surviving meristem regenerated a single shoot within two to three months after thawing.
引用
收藏
页码：405 / 412
页数：8
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