CLONING AND SEQUENCING A CDNA-ENCODING HUMAN RIBOSOMAL PROTEIN-S25

被引：13

作者：

LI, ML ^{[1
]}

LATOUD, C ^{[1
]}

CENTER, MS ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI,COLL BIOL,CTR BASIC CANC RES,MANHATTAN,KS 66506

来源：

GENE | 1991年 / 107卷 / 02期

关键词：

RECOMBINANT DNA; SUBTRACTIVE CDNA LIBRARY; SYNTHETIC PEPTIDE; CONSERVED SEQUENCE;

D O I：

10.1016/0378-1119(91)90335-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A full-length cDNA clone has been isolated from a cDNA library prepared from mRNA of adriamycin-resistant human leukemia HL60 cells. The nucleotide sequence of this cDNA has been determined and the protein coded for by the gene identified. The cDNA encodes a polypeptide of 125 amino acids (aa) with a deduced M(r) of 13750. The deduced aa sequence of this protein has 56% homology to yeast ribosomal protein S31. Western-blot analysis using antibodies directed against a synthetic peptide based on the deduced aa sequence identifies the gene product as the human ribosomal protein S25.

引用

页码：329 / 333

页数：5

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