MUTANTS OF SACCHAROMYCES-CEREVISIAE DEFECTIVE IN THE FARNESYLATION OF RAS PROTEINS

被引：111
作者：
GOODMAN, LE
JUDD, SR
FARNSWORTH, CC
POWERS, S
GELB, MH
GLOMSET, JA
TAMANOI, F
机构：
[1] UNIV CHICAGO,DEPT BIOCHEM & MOLEC BIOL,920 E 58TH ST,CHICAGO,IL 60637
[2] UNIV WASHINGTON,HOWARD HUGHES MED INST LAB,SEATTLE,WA 98195
[3] UNIV WASHINGTON,DEPT MED & BIOCHEM,SEATTLE,WA 98195
[4] UNIV WASHINGTON,DEPT BIOCHEM,SEATTLE,WA 98195
[5] UNIV WASHINGTON,DEPT CHEM,SEATTLE,WA 98195
[6] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT BIOCHEM,PISCATAWAY,NJ 08854
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 24期
关键词：
CAAX" box; C-terminal processing; Farnesyltransferase;
D O I：
10.1073/pnas.87.24.9665
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Ras proteins are post-translationally modified by farnesylation. In the present investigation, we identified an activity in crude soluble extracts of yeast cells that catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to yeast RAS2 protein. RAS2 proteins having a C-terminal Cys-Ali-Ali-Xaa sequence (where Ali is an aliphatic amino acid and Xaa is the unspecified C-terminal amino acid) served as substrates for this reaction, whereas RAS2 proteins with an altered or deleted Cys-Ali-Ali-Xaa sequence did not. A yeast mutant, dpr1/ram1, originally isolated as a Ras-processing mutant was shown to be defective in farnesyltransferase activity. In addition, another mutant, ram2, also was defective in the transferase activity. These results demonstrate that at least two genes, DPR1/RAM1 and RAM2, are required for the farnesyltransferase activity in yeast. (.
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页码：9665 / 9669
页数：5
相关论文
共 26 条
[1]   DIFFERENTIAL ACTIVATION OF YEAST ADENYLATE-CYCLASE BY WILD-TYPE AND MUTANT RAS PROTEINS [J].
BROEK, D ;
SAMIY, N ;
FASANO, O ;
FUJIYAMA, A ;
TAMANOI, F ;
NORTHUP, J ;
WIGLER, M .
CELL, 1985, 41 (03) :763-769
[2]   P21RAS IS MODIFIED BY A FARNESYL ISOPRENOID [J].
CASEY, PJ ;
SOLSKI, PA ;
DER, CJ ;
BUSS, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8323-8327
[3]   POSTTRANSLATIONAL MODIFICATION OF THE HA-RAS ONCOGENE PROTEIN - EVIDENCE FOR A 3RD CLASS OF PROTEIN CARBOXYL METHYLTRANSFERASES [J].
CLARKE, S ;
VOGEL, JP ;
DESCHENES, RJ ;
STOCK, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4643-4647
[4]  
DAVISSON VJ, 1986, BIOORG CHEM, V14, P46
[5]  
FARNSWORTH CC, 1989, J BIOL CHEM, V264, P20422
[6]   IDENTIFICATION OF GERANYLGERANYL-MODIFIED PROTEINS IN HELA-CELLS [J].
FARNSWORTH, CC ;
GELB, MH ;
GLOMSET, JA .
SCIENCE, 1990, 247 (4940) :320-322
[7]   COMMON MODIFICATIONS OF TRIMERIC-G PROTEINS AND RAS PROTEIN - INVOLVEMENT OF POLYISOPRENYLATION [J].
FINEGOLD, AA ;
SCHAFER, WR ;
RINE, J ;
WHITEWAY, M ;
TAMANOI, F .
SCIENCE, 1990, 249 (4965) :165-169
[8]   PROCESSING AND FATTY-ACID ACYLATION OF RAS1 AND RAS2 PROTEINS IN SACCHAROMYCES-CEREVISIAE [J].
FUJIYAMA, A ;
TAMANOI, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (05) :1266-1270
[9]   A NOVEL YEAST MUTANT DEFECTIVE IN THE PROCESSING OF RAS PROTEINS - ASSESSMENT OF THE EFFECT OF THE MUTATION ON PROCESSING STEPS [J].
FUJIYAMA, A ;
MATSUMOTO, K ;
TAMANOI, F .
EMBO JOURNAL, 1987, 6 (01) :223-228
[10]  
FUJIYAMA A, 1990, J BIOL CHEM, V265, P3362
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