DEVELOPMENT OF AN ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF METAZACHLOR

被引：10
|
作者：
SCHOLZ, HM
HOCK, B
机构：
[1] Technische Universitlt Miinchen at Weihenstephan, Department of Botany, 8050, Freising
来源：
ANALYTICAL LETTERS | 1991年 / 24卷 / 03期
关键词：
METAZACHLOR; EIA; ELISA; ENZYME IMMUNOASSAY; SOLID PHASE; HERBICIDE; POLYCLONAL ANTIBODIES;
D O I：
10.1080/00032719108052915
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sensitive, solid phase microtitre plate enzyme immunoassay (ELISA) was developed for the determination of the herbicide metazachlor, which belongs to the class of alpha-chloroacetamides. The antisera were raised in rabbits by immunization with a metazachlor-BSA conjugate containing 27 metazachlor residues per molecule. A competitive ELISA with a peroxidase-metazachlor tracer was performed. Metazachlor could be detected within the range of 0.01 and 1-mu-g/l. The test showed no cross-reactivity with other structurally similar chloroacetamide herbicides and little cross-reactivity with derivatives of metazachlor.
引用
收藏
页码：413 / 427
页数：15
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