HLA CLASS-I TYPING BY PCR - HLA-B27 AND AN AFRICAN B27 SUBTYPE

被引：152

作者：

HILL, AVS ^{[1
]}

ALLSOPP, CEM ^{[1
]}

KWIATKOWSKI, D ^{[1
]}

ANSTEY, NM ^{[1
]}

GREENWOOD, BM ^{[1
]}

MCMICHAEL, AJ ^{[1
]}

机构：

[1] MRC LABS,FAJARA,SENEGAMBIA

来源：

LANCET | 1991年 / 337卷 / 8742期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0140-6736(91)92452-8

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We describe a rapid method of HLA class I typing using the polymerase chain reaction and oligonucleotide hybridisation that eliminates the requirements for viable lymphocytes and allows subtypes to be defined. We have used this to demonstrate that the predominant subtype of HLA-B27 in the Gambia, West Africa, is HLA-B*2703, which is very rare or absent in other racial groups. This subtype differs from the common Caucasian HLA-B27 subtypes in its recognition by cytotoxic T cells. We propose that HLA*B-2703, unlike other HLA-B27 subtypes, may not be associated with ankylosing spondylitis, thus accounting in part for the rarity of this condition in black populations.

引用

页码：640 / 642

页数：3

共 24 条

[1] SEQUENCE-ANALYSIS OF HLA-BW53, A COMMON WEST AFRICAN ALLELE, SUGGESTS AN ORIGIN BY GENE CONVERSION OF HLA-B35 [J].