MAINTENANCE OF ENERGY-LINKED FUNCTIONS IN RAT-LIVER MITOCHONDRIA

被引：7

作者：

LUND, P

WIGGINS, D

机构：

[1] Metabolic Research Laboratory, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1018卷 / 01期

关键词：

(Rat liver mitochondria); Aging; Citrulline synthesis; EDTA; EGTA; Pyruvate carboxylation;

D O I：

10.1016/0005-2728(90)90115-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

EGTA and EDTA are compared with respect to their ability to preserve ATP-requiring reactions in rat liver mitochondria. The presence of EGTA sustains the high ATP requirement of citrulline synthesis. EDTA does not, even with excess Mg2+. Carboxylation of pyruvate, which has a lower ATP demand, is not influenced by the type of chelating agent. In mitochondria stored for 24 h at 4° C, EGTA is more effective than EDTA in preventing loss of these energy-linked functions. © 1990.

引用

页码：98 / 102

页数：5

共 25 条

[1] SWELLING AND SHRINKAGE PHENOMENA IN LIVER MITOCHONDRIA .2. LOW AMPLITUDE SWELLING-SHRINKAGE CYCLES [J].