HOMOGENEITY AND SEQUENTIAL PERIODATE OXIDATION OF A GLYCOPEPTIDE FROM TAKA-AMYLASE A

被引：26

作者：

YAMAGUCHI, H

IKENAKA, T

MATSUSHIMA, Y

机构：

[1] Department of Chemistry, Osaka University College of Science, Toyonaka

来源：

JOURNAL OF BIOCHEMISTRY | 1969年 / 65卷 / 05期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a129079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The homogeneity of a glycopeptide obtained from Taka-amylase A [EC 3.2.1.1] was reinvestigated by column chromatography on Dowex 50×2 and high voltage paper electrophoresis. About 90 per cent of the glycopeptide was found to be homogeneous and to consist of one mole each of aspartic acid, serine and ammonia, 2 moles of N-acetylglucosamine and 6 moles of mannose.Sequential pcriodate oxidation of the purified homogeneous glycopeptide indicated that its carbohydrate sequence is Man-Man-GlcNAc-GlcNAc-NH. © 1969 BY THE JOURNAL OF BIOCHEMISTRY.

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页码：793 / +

页数：1

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