PHYSIOLOGICAL CYTOSOLIC CA2+ TRANSIENTS EVOKE CONCURRENT MITOCHONDRIAL DEPOLARIZATIONS

被引：124

作者：

LOEW, LM ^{[1
]}

CARRINGTON, W ^{[1
]}

TUFT, RA ^{[1
]}

FAY, FS ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS, SCH MED, DEPT PHYSIOL, BIOMED IMAGING GRP, WORCESTER, MA 01655 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 26期

关键词：

MEMBRANE POTENTIAL; FLUORESCENCE; BIOENERGETICS; IMAGE PROCESSING; MICROSCOPY;

D O I：

10.1073/pnas.91.26.12579

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Calcium, a ubiquitous second messenger, stimulates the activity of several mitochondrial dehydrogenases. This has led to the suggestion that the same messenger that signals cell activation could also activate mitochondrial electron/proton transport, thereby meeting demands for increased cellular energy. To test this in live cells, quantitative three-dimensional microscopy and ratio imaging were used to measure membrane potential of individual mitochondria and cytosolic calcium distribution. Mitochondria reversibly depolarized as cytosolic calcium rose and then fell following physiological stimulation. Thus, the dominant response of the mitochondrion to a rise in cytosolic [Ca2+] is to draw on the electrochemical potential, possibly to accelerate processes directly involved in ATP synthesis and calcium homeostasis.

引用

页码：12579 / 12583

页数：5

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