IDENTIFICATION OF NEUTRALIZING ANTIGENIC SITES ON VP1 AND VP2 OF TYPE-A5 FOOT-AND-MOUTH-DISEASE VIRUS, DEFINED BY NEUTRALIZATION-RESISTANT VARIANTS

被引:64
作者
SAIZ, JC
GONZALEZ, MJ
BORCA, MV
SOBRINO, F
MOORE, DM
机构
[1] USDA ARS,PLUM ISL ANIM DIS CTR,MOLEC BIOL LAB,GREENPORT,NY 11944
[2] INST NACL INVEST AGR,DEPT SANIDAD ANIM,E-28012 MADRID,SPAIN
关键词
D O I
10.1128/JVI.65.5.2518-2524.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Five neutralizing monoclonal antibodies (nMAbs) obtained against type A5 Spain-86 foot-and-mouth disease virus were used to generate a series of neutralization-resistant variants. In vitro and in vivo assays showed that the variants were fully refractory to neutralization by the selecting nMAb. On the basis of cross-neutralization and binding assays, two neutralizing antigenic sites have been located on the virus surface; one, located near the C-terminus of VP1, displayed a linear epitope, and the second, located on VP2, displayed two conformational epitopes. Nucleotide sequencing of RNA of the parental and variant capsid protein-coding region P1 has placed the amino acid changes at position 198 of VP1 for the first site and at positions 72 and 79 of VP2 for the related epitopes in the second site. The relative importance of these two sites in the biological properties of foot-and-mouth disease virus is discussed.
引用
收藏
页码:2518 / 2524
页数:7
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