HYBRIDOMA GROWTH, METABOLISM, AND PRODUCT FORMATION IN HEPES-BUFFERED MEDIUM .1. EFFECT OF PASSAGE NUMBER

被引：11

作者：

SCHMID, G ^{[1
]}

BLANCH, HW ^{[1
]}

WILKE, CR ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT CHEM ENGN,BERKELEY,CA 94720

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 09期

关键词：

D O I：

10.1007/BF01088184

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Shake flask studies with a murine hybridoma cell line show drastically increased cell yields per mL of culture medium as function of passage number ("cell age"), whereas the maximum antibody concentrations at the end of batch growth decreased from 180 μg/mL to 75 μg/mL after 10 months of serial propagation in flasks. The results are presented in the light of differences in cell metabolism with glucose, lactate, ammonia, glutamine, and selected amino acids being monitored. © 1990 Kluwer Academic Publishers.

引用

页码：627 / 632

页数：6

共 14 条

[1]

BUNTEMEYER H, 1988, THESIS U HANNOVER FR

[2]

BUTLER M, 1983, J CELL SCI, V61, P351

[3]

CUSTER L, 1987, UNPUB

[4]

DITTMAR KEJ, 1984, 3RD C P EUR C BIOT, V1, P183