EFFECT OF INTERLEUKIN-1 ON HYALURONATE SYNTHESIS BY SYNOVIAL FIBROBLASTIC CELLS

被引：15
作者：
KONTTINEN, YT
SAARI, H
NORDSTROM, DC
机构：
[1] Division of Rheumatology, Fourth Department of Medicine, Helsinki University Central Hospital, Helsinki
来源：
CLINICAL RHEUMATOLOGY | 1991年 / 10卷 / 02期
关键词：
INTERLEUKIN-1; FIBROBLAST; HYALURONATE;
D O I：
10.1007/BF02207654
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Interleukin-1 (IL-1) stimulates fibroblast-mediated hyaluronate (HA) synthesis in vitro. In the present study the degree of polymerization of such HA was studied using HPLC (high performance liquid chromatography) with a size exclusion column combined with I-125-HABP assay used to measure the HA concentration in various HA molecular weight fractions separated using HPLC. IL-1 stimulated HA was more polydisperse than that produced by resting fibroblasts with a molecular weight varying from more than 4 x 10(6) daltons to less than 7.1 x 10(3) daltons. This IL-1 effect may contribute to the low molecular weight HA produced by freshly explanted arthritic synovial tissue and to the low viscosity of arthritic synovial fluid in vivo.
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收藏
页码：151 / 154
页数：4
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  • [1] Laurent T.C., Fraser R.E., The properties and turnover of hyaluronan, Ciba Foundation Symp, 124, pp. 9-29, (1986)
  • [2] Blau S., Janis R., Hamerman D., Sandson J., Cellular origin of hyaluronateprotein in the human synovial membrane, Science, 150, pp. 353-355, (1965)
  • [3] Konttinen Y.T., Saari H., Santavirta S., Antti-Poika I., Sorsa T., Nykanen P., Kemppinen P., Synovial fibroblasts, Scand J Rheumatol, pp. 95-103, (1988)
  • [4] Bjelle A., Andersson T., Granath K., Molecular weight distribution of hyaluronic acid of human synovial fluid in rheumatic diseases, Scand J Rheumatol, 12, pp. 133-138, (1982)
  • [5] Dahl L.B., Dahl I.M.S., Engstrom-Laurent A., Granath K., Concentration and molecular weight of sodium hyaluronate in synovial fluid from patients with rheumatoid arthritis and other arthropathies, Ann Rheum Dis, 44, pp. 817-822, (1985)
  • [6] Saari H., Konttinen Y.T., Determination of the concentration and polymerization of synovial fluid hyaluronate using high performance liquid chromatography, Ann Rheum Dis, 48, pp. 565-570, (1989)
  • [7] Dahl I.M.S., Husby G., Hyaluronic acid production in vitro by synovial lining cells from normal and rheumatoid joints, Ann Rheum Dis, 44, pp. 647-657, (1985)
  • [8] Konttinen Y.T., Nykanen P., Nordstrom D., Saari H., Sandelin J., Santavirta S., Kouri T., DNA synthesis in prolyl 4-hydroxylase positive fibroblasts in situ in synovial tissue. An autoradiographyimmunoperoxidase double labeling study, J Rheumatol, 16, pp. 339-345, (1989)
  • [9] Konttinen Y.T., von Essen R., Gronblad M., Vahvanen V., Santavirta S., Antti-Poika I., Hamalainen M., Fibroblasts in synovitis in rheumatoid arthritis but not in non-inflammatory synovial tissue in meniscus lesions express the carboxyterminal domain of procollagen type I, Am J Clin Pathol, 93, pp. 340-346, (1990)
  • [10] Greenwald R.A., Moy W.W., Effect of oxygen-derived free radicals on hyaluronic acid, Arthritis Rheum, 23, pp. 455-463, (1980)
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