CORRELATIVE LIGHT-MICROSCOPY, SCANNING ELECTRON-MICROSCOPY, AND TRANSMISSION ELECTRON-MICROSCOPY OF OSMIUM-MACERATED BIOLOGICAL TISSUES

被引：0

作者：

SCALA, C ^{[1
]}

CENACCHI, G ^{[1
]}

APKARIAN, RP ^{[1
]}

PREDA, P ^{[1
]}

PASQUINELLI, G ^{[1
]}

机构：

[1] EMORY UNIV,YERKES REG PRIMATE RES CTR,ATLANTA,GA 30322

来源：

JOURNAL OF ELECTRON MICROSCOPY | 1990年 / 39卷 / 06期

关键词：

OSMIC MACERATION; SEMITHIN SECTIONS; PLASTIC REMOVAL; 3-DIMENSIONAL MICROANATOMY; LM-TEM-SEM CORRELATIONS;

D O I：

暂无

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A method facilitating correlation of light microscopic (LM), scanning electron microscopic (SEM) and transmission electron microscopic (TEM) images was developed. Rat kidney and heart were initially subjected to the osmium maceration procedure and then embedded in acrylic resin. Semithin sections of the tissue blocks were first provided for LM and then examined by SEM after resin removal. Furthermore, the ultrathin sections adjacent to the semithin sections were observed by TEM. The three-dimensional images of intracellular organelles provided an informative adjunct to LM and TEM.

引用

页码：508 / 510

页数：3

共 6 条

[1] CONVENTIONAL AND HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPY OF BIOLOGICAL SECTIONED MATERIAL
SCALA, C
CENACCHI, G
PREDA, P
VICI, M
APKARIAN, RP
PASQUINELLI, G
SCANNING MICROSCOPY, 1991, 5 (01) : 135 - 145
[2] APPLICATION OF AN OSMIUM-MACERATION TECHNIQUE TO OBSERVE PLANT MICROBE INTERFACES OF ITALIAN RYEGRASS AND CROWN RUST FUNGI BY SCANNING ELECTRON-MICROSCOPY
KOGA, H
TSUKIBOSHI, T
UEMATSU, T
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[3] SCANNING ELECTRON-MICROSCOPY OF CHRONIC HEPATITIS-C - AN OSO4 MACERATION STUDY ON HUMAN BIOPSIES
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AMBU, R
CONGIU, T
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COSTA, V
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[4] Scanning electron microscopy in liver biopsy interpretation in children: a mini atlas
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[5] Three-sectioning method: A procedure for studying hard tissues and large pieces under light and electron microscopy
Gayoso, Jorge
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Alberto Rodriguez-Arias, Carlos
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MICRON, 2020, 132
[6] Neuroanatomy from Mesoscopic to Nanoscopic Scales: An Improved Method for the Observation of Semithin Sections by High-Resolution Scanning Electron Microscopy
Rodriguez, Jose-Rodrigo
Turegano-Lopez, Marta
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FRONTIERS IN NEUROANATOMY, 2018, 12

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