CORRELATIVE LIGHT-MICROSCOPY, SCANNING ELECTRON-MICROSCOPY, AND TRANSMISSION ELECTRON-MICROSCOPY OF OSMIUM-MACERATED BIOLOGICAL TISSUES

被引：0

作者：

SCALA, C ^{[1
]}

CENACCHI, G ^{[1
]}

APKARIAN, RP ^{[1
]}

PREDA, P ^{[1
]}

PASQUINELLI, G ^{[1
]}

机构：

[1] EMORY UNIV,YERKES REG PRIMATE RES CTR,ATLANTA,GA 30322

来源：

JOURNAL OF ELECTRON MICROSCOPY | 1990年 / 39卷 / 06期

关键词：

OSMIC MACERATION; SEMITHIN SECTIONS; PLASTIC REMOVAL; 3-DIMENSIONAL MICROANATOMY; LM-TEM-SEM CORRELATIONS;

D O I：

暂无

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A method facilitating correlation of light microscopic (LM), scanning electron microscopic (SEM) and transmission electron microscopic (TEM) images was developed. Rat kidney and heart were initially subjected to the osmium maceration procedure and then embedded in acrylic resin. Semithin sections of the tissue blocks were first provided for LM and then examined by SEM after resin removal. Furthermore, the ultrathin sections adjacent to the semithin sections were observed by TEM. The three-dimensional images of intracellular organelles provided an informative adjunct to LM and TEM.

引用

页码：508 / 510

页数：3

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