METHIONYL-TRANSFER RNA-SYNTHETASE FROM SHEEP LIVER - PURIFICATION OF A FULLY ACTIVE MONOMER DERIVED FROM HIGH-MOLECULAR-WEIGHT COMPLEXES BY TRYPSIN TREATMENT - EVIDENCE FOR IMMUNOLOGICAL CROSS-REACTION WITH CORRESPONDING ENZYME FROM SHEEP MAMMARY-GLAND

被引：34
作者：
KELLERMANN, O
BREVET, A
TONETTI, H
WALLER, JP
机构：
[1] Laboratoire de Biochimie, Ecole Polytechnique, Palaiseau
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1978年 / 88卷 / 01期
关键词：
D O I：
10.1111/j.1432-1033.1978.tb12439.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The size distribution of methionyl‐tRNA synthetase in extracts from sheep liver is compared to that of lysyl‐tRNA, isoleucyl‐tRNA, leucyl‐tRNA and seryl‐tRNA synthetases by gel filtration on Biogel A‐5m. Extraction conditions are described which lead to isolation of methionyl‐tRNA synthetase exclusively in the form of complexes of molecular weight close to 106. Limited trypsin treatment of these aggregates releases a fully active low‐molecular‐weight form of methionyl‐tRNA synthetase which was purified to a specific activity of 674 units/mg at 25 °C with a yield of 40%. The homogeneous enzyme appears to be undistinguishable from the corresponding enzyme derived from sheep lactating mammary gland, as judged by acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and by titration with antibodies raised against the enzyme purified from liver. Copyright © 1978, Wiley Blackwell. All rights reserved
引用
收藏
页码：205 / 210
页数：6
相关论文
共 11 条
[1]  
Anfinsen C B, 1975, Adv Protein Chem, V29, P205, DOI 10.1016/S0065-3233(08)60413-1
[2]   MODIFICATION OF METHIONYL-TRNA SYNTHETASE BY PROTEOLYTIC CLEAVAGE AND PROPERTIES OF TRYPSIN-MODIFIED ENZYME [J].
CASSIO, D ;
WALLER, JP .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1971, 20 (02) :283-+
[3]  
CLAUSEN J, 1969, IMMUNOLOGICAL TECH 3, V1
[4]   DETECTION AND PARTIAL-PURIFICATION OF RAPIDLY SEDIMENTING FORMS OF AMINOACYL TRANSFER RIBONUCLEIC-ACID SYNTHETASES FROM HUMAN PLACENTA [J].
DENNEY, RM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 183 (01) :156-167
[5]   PURIFICATION OF TRANSFER-RNA (TRP, TRANSFER-RNA (VAL, AND PARTIAL-PURIFICATION OF TRANSFER-RNA (ILE) AND TRANSFER-RNA (F)MET FROM BEEF-LIVER [J].
FOURNIER, M ;
DORIZZI, M ;
SARGER, C ;
LABOUESSE, J .
BIOCHIMIE, 1976, 58 (10) :1159-1165
[6]   OCCURRENCE OF A COMPLEX OF AMINOACYL-TRANSFER-RNA SYNTHETASES IN LACTATING RAT MAMMARY-GLAND [J].
HELE, P ;
HEBERT, L .
BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 479 (03) :311-321
[7]   METHIONYL-TRANSFER RNA-SYNTHETASE FROM SHEEP MAMMARY-GLAND - PURIFICATION OF A FULLY ACTIVE MONOMERIC ENZYME DERIVED FROM HIGH-MOLECULAR-WEIGHT COMPLEXES BY CONTROLLED PROTEOLYSIS [J].
KELLERMANN, O ;
VIEL, C ;
WALLER, JP .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 88 (01) :197-204
[8]   SENSITIVE DOUBLE ANTIBODY IMMUNOASSAY FOR HUMAN GROWTH HORMONE IN PLASMA [J].
SCHALCH, DS ;
PARKER, ML .
NATURE, 1964, 203 (495) :1141-&
[9]   ISOLATION AND PARTIAL CHARACTERIZATION OF AN AMINOACYL-TRANSFER-RNA SYNTHETASE COMPLEX FROM RABBIT RETICULOCYTES [J].
SOM, K ;
HARDESTY, B .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 166 (02) :507-517
[10]  
TWARDOWSKI T, 1976, BIOCHEM BIOPH RES CO, V71, P272
12