PROHORMONE PROCESSING BY YEAST PROTEASES

被引：16

作者：

BOURBONNAIS, Y

GERMAIN, D

LATCHINIANSADEK, L

BOILEAU, G

THOMAS, DY

机构：

[1] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,EUKARYOT GENET GRP,MONTREAL H4P 2R2,QUEBEC,CANADA

[2] UNIV MONTREAL,DEPT BIOCHIM,MONTREAL H3C 3J7,QUEBEC,CANADA

[3] MCGILL UNIV,DEPT BIOL,MONTREAL H3A 2T5,QUEBEC,CANADA

来源：

ENZYME | 1991年 / 45卷 / 5-6期

关键词：

YEAST; PROTEASE; KEX2; KEX1; PROCESSING; PROHORMONE;

D O I：

10.1159/000468899

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Investigations of the precursors of alpha-pheromone and killer toxin in the yeast Saccharomyces cerevisiae have defined the genes coding (KEX1 and KEX2) for the proteases which are responsible for their processing. In addition to processing at pairs of basic residues it is evident that yeast can also process at monobasic sites. We present data on the Kex1p and Kex2p enzymes, their cellular localization, and their post-translational modification. In addition initial characterisation of the monobasic specific protease and the isolation of mutants defective in this activity are presented. The use of the yeast system as a model for the processing of mammalian prohormones is discussed.

引用

页码：244 / 256

页数：13

共 46 条

[1] HORMONE PROCESSING AND MEMBRANE-BOUND PROTEINASES IN YEAST [J].