HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF EQUINE ESTROGENS WITH ULTRAVIOLET ABSORBENCY AND ELECTROCHEMICAL DETECTION

被引：9
|
作者：
NOVAKOVIC, J
TVRZICKA, E
PACAKOVA, V
机构：
[1] CHARLES UNIV,FAC MED 1,DEPT MED 4,CR-12808 PRAGUE 2,CZECH REPUBLIC
[2] CHARLES UNIV,FAC NAT SCI,DEPT ANALYT CHEM,CR-12840 PRAGUE 2,CZECH REPUBLIC
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 678卷 / 02期
关键词：
D O I：
10.1016/0021-9673(94)80484-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The simultaneous separation of a mixture of equine estrogens containing estrone, equilin, equilenin and their corresponding 17 alpha-diols was accomplished by reversed-phase high-performance liquid chromatography (RP-HPLC) on a Nucleosil C-18 column with a mobile phase composed of methanol-water-2-propanol-dichloromethane (45:42.5:7.5:5, v/v). UV absorbance and electrochemical detection were used for the analysis of a standard mixture of equine estrogens. RP-HPLC with UV absorbance detection was applied to the determination of these compounds in pharmaceutical drug substances and drug products after acid hydrolysis of their sodium sulfate esters.
引用
收藏
页码：359 / 363
页数：5
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