HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF EQUINE ESTROGENS WITH ULTRAVIOLET ABSORBENCY AND ELECTROCHEMICAL DETECTION

被引：9
|
作者：
NOVAKOVIC, J
TVRZICKA, E
PACAKOVA, V
机构：
[1] CHARLES UNIV,FAC MED 1,DEPT MED 4,CR-12808 PRAGUE 2,CZECH REPUBLIC
[2] CHARLES UNIV,FAC NAT SCI,DEPT ANALYT CHEM,CR-12840 PRAGUE 2,CZECH REPUBLIC
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 678卷 / 02期
关键词：
D O I：
10.1016/0021-9673(94)80484-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The simultaneous separation of a mixture of equine estrogens containing estrone, equilin, equilenin and their corresponding 17 alpha-diols was accomplished by reversed-phase high-performance liquid chromatography (RP-HPLC) on a Nucleosil C-18 column with a mobile phase composed of methanol-water-2-propanol-dichloromethane (45:42.5:7.5:5, v/v). UV absorbance and electrochemical detection were used for the analysis of a standard mixture of equine estrogens. RP-HPLC with UV absorbance detection was applied to the determination of these compounds in pharmaceutical drug substances and drug products after acid hydrolysis of their sodium sulfate esters.
引用
收藏
页码：359 / 363
页数：5
相关论文
共 50 条
  • [1] SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ADENOSINE AND DOPAMINE IN RAT STRIATAL TISSUE WITH COMBINED ULTRAVIOLET ABSORBENCY AND ELECTROCHEMICAL DETECTION
    BETTO, P
    POPOLI, P
    RICCIARELLO, G
    CAPORALI, MG
    ANTONINI, R
    JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 662 (01): : 21 - 25
  • [2] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CONJUGATED ESTROGENS IN TABLETS
    TOWNSEND, RW
    KEUTH, V
    EMBIL, K
    MULLERSMAN, G
    PERRIN, JH
    DERENDORF, H
    JOURNAL OF CHROMATOGRAPHY, 1988, 450 (03): : 414 - 419
  • [3] IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMAMANITINS WITH ELECTROCHEMICAL DETECTION
    TAGLIARO, F
    CHIMINAZZO, S
    MASCHIO, S
    ALBERTON, F
    MARIGO, M
    CHROMATOGRAPHIA, 1987, 24 : 482 - 486
  • [4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF DEOXYSPERGUALIN IN DOG PLASMA WITH ULTRAVIOLET DETECTION
    NAKANUMA, R
    WATANABE, K
    YAMASHITA, K
    MIZUGUCHI, S
    HASHIMOTO, Y
    NAKAMURA, T
    UMEZAWA, H
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 527 (01): : 208 - 213
  • [5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BUSULFAN IN HUMAN SERUM WITH ONLINE DERIVATIZATION, COLUMN-SWITCHING AND ULTRAVIOLET ABSORBENCY DETECTION
    FUNAKOSHI, K
    YAMASHITA, K
    CHAO, WF
    YAMAGUCHI, M
    YASHIKI, T
    JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 660 (01): : 200 - 204
  • [6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ESTROGENS IN HUMAN-URINE
    CARTONI, GP
    COCCIOLI, F
    JOURNAL OF CHROMATOGRAPHY, 1983, 278 (01): : 144 - 150
  • [7] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE AND ITS DERIVATIVES WITH ELECTROCHEMICAL DETECTION
    GREENBERG, MS
    MAYER, WJ
    JOURNAL OF CHROMATOGRAPHY, 1979, 169 (FEB): : 321 - 327
  • [8] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CYMIAZOLE IN HONEY WITH UV AND ELECTROCHEMICAL DETECTION
    GOMIS, DB
    FERNANDEZ, AC
    BERNARDO, VM
    ALVAREZ, MDG
    CHROMATOGRAPHIA, 1994, 39 (9-10) : 602 - 606
  • [9] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ETOPOSIDE IN PLASMA USING ELECTROCHEMICAL DETECTION
    LITTLEWOOD, TJ
    HUTCHINGS, AL
    BENTLEY, DP
    SPRAGG, BP
    JOURNAL OF CHROMATOGRAPHY, 1984, 336 (02): : 434 - 437
  • [10] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF EUGENOL IN PIMENTO USING ULTRAVIOLET AND ELECTROCHEMICAL DETECTION
    SMITH, RM
    BECK, S
    JOURNAL OF CHROMATOGRAPHY, 1984, 291 (MAY): : 424 - 427
12345