ENDONUCLEASE-VII OF PHAGE-T4 TRIGGERS MISMATCH CORRECTION INVITRO

被引：72

作者：

SOLARO, PC ^{[1
]}

BIRKENKAMP, K ^{[1
]}

PFEIFFER, P ^{[1
]}

KEMPER, B ^{[1
]}

机构：

[1] UNIV COLOGNE,INST GENET,WEYERTAL 121,W-5000 COLOGNE 41,GERMANY

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 03期

关键词：

BACTERIOPHAGE-T4; ENDONUCLEASE-VII; HETERODUPLEX DNA; MISMATCH CORRECTION; OLIGONUCLEOTIDES;

D O I：

10.1006/jmbi.1993.1207

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reactivity of endonuclease VII (gp49 of phage T4) with DNA-loops of eight, four, or one nucleotide, or any of 12 possible base mismatches was tested in vitro. Endonuclease VII introduces double-strand breaks by nick and counter-nick within six nucleotides 3′ from the mispairings. High relative cleavage efficiencies at mismatches in heteroduplexes correlate with their decreased thermal stability and vice versa. A delay between nick and counter-nick was sufficient to allow T4 DNA-polymerase and T4 DNA-ligase to correct a C/C-mismatch in vitro, thereby saving the DNA from double-strand breakage. Very short repair tracks of three to four nucleotides mapped between the mismatch and one of the formerly induced nicks, which were subsequently sealed by DNA ligase. © 1993 Academic Press, Inc.

引用

页码：868 / 877

页数：10