PRODUCTION OF HUMAN MATRIX METALLOPROTEINASE-3 (STROMELYSIN) IN ESCHERICHIA-COLI

被引：17

作者：

ROSENFELD, SA ^{[1
]}

ROSS, OH ^{[1
]}

CORMAN, JI ^{[1
]}

PRATTA, MA ^{[1
]}

BLESSINGTON, DL ^{[1
]}

FEESER, WS ^{[1
]}

FREIMARK, BD ^{[1
]}

机构：

[1] DUPONT MERCK PHARMACEUT CO, INFLAMMATORY DIS RES, WILMINGTON, DE 19880 USA

来源：

GENE | 1994年 / 139卷 / 02期

关键词：

RECOMBINANT DNA; INCLUSION BODIES; PROTEIN SOLUBILIZATION; PROTEIN REFOLDING; PURIFICATION; ENZYMATIC ACTIVITY;

D O I：

10.1016/0378-1119(94)90770-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Full-length human matrix metalloproteinase 3 (prostromelysin or proMMP-3) was produced in Escherichia coli as an intracellular insoluble aggregate that could be solublized and refolded to yield an activatable proenzyme. The refolded protein was purified to >95% homogeneity. The recombinant proMMP-3 (re-proMMP-3) could be activated by agents known to stimulate self-catalyzed cleavage of native fibroblast proMMP-3. The N-terminal amino-acid sequence of the re-proMMP-3 and its activation products indicated that they were the same as those obtained with the natural material.

引用

页码：281 / 286

页数：6

共 50 条

[41] Identification of matrix metalloproteinase-3 (MMP-3 or stromelysin-1) cleavage products of fibrin(ogen) in human synovial fluid.
Bini, A
Wu, D
Ivashkiv, L
Ahadi, MZ
Kudryk, BJ
FASEB JOURNAL, 1999, 13 (04): : A206 - A206
[42] Histamine stimulates matrix metalloproteinase-3 and-13 production by human articular chondrocytes in vitro
Tetlow, LC
Woolley, DE
ANNALS OF THE RHEUMATIC DISEASES, 2002, 61 (08) : 737 - 740
[43] DISRUPTION OF THE CYSTEINE-75 AND ZINC ION COORDINATION IS NOT SUFFICIENT TO ACTIVATE THE PRECURSOR OF HUMAN MATRIX METALLOPROTEINASE-3 (STROMELYSIN-1)
CHEN, LC
NOELKEN, ME
NAGASE, H
BIOCHEMISTRY, 1993, 32 (39) : 10289 - 10295
[44] IMMUNOLOCALIZATION OF MATRIX METALLOPROTEINASE-3 (STROMELYSIN) IN RHEUMATOID SYNOVIOBLASTS (B-CELLS) - CORRELATION WITH RHEUMATOID-ARTHRITIS
OKADA, Y
TAKEUCHI, N
TOMITA, K
NAKANISHI, I
NAGASE, H
ANNALS OF THE RHEUMATIC DISEASES, 1989, 48 (08) : 645 - 653
[45] STEPWISE ACTIVATION MECHANISMS OF THE PRECURSOR OF MATRIX METALLOPROTEINASE-3 (STROMELYSIN) BY PROTEINASES AND (4-AMINOPHENYL)MERCURIC ACETATE
NAGASE, H
ENGHILD, JJ
SUZUKI, K
SALVESEN, G
BIOCHEMISTRY, 1990, 29 (24) : 5783 - 5789
[46] PRODUCTION OF HUMAN PROINSULIN BY IMMOBILIZED ESCHERICHIA-COLI
BIRNBAUM, S
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1988, 196 : 74 - MBTD
[47] PRODUCTION OF RECOMBINANT HUMAN CALCITONIN IN ESCHERICHIA-COLI
YABUTA, M
SUZUKI, Y
MAGOTA, K
TSURUOKA, N
MATSUKURA, S
TANAKA, S
OHSHIMA, T
OHSUYE, K
RECOMBINANT DNA TECHNOLOGY II, 1994, 721 : 82 - 84
[48] PRODUCTION OF HUMAN SERUM TRANSFERRIN IN ESCHERICHIA-COLI
IKEDA, RA
BOWMAN, BH
YANG, FM
LOKEY, LK
GENE, 1992, 117 (02) : 265 - 269
[49] PRODUCTION OF MATURE HUMAN STATHERIN ESCHERICHIA-COLI
CHOI, YJ
TRACKMAN, PC
TROXLER, RT
OPPENHEIM, FG
JOURNAL OF DENTAL RESEARCH, 1995, 74 : 196 - 196
[50] ROCK inhibition enhances aggrecan deposition and suppresses matrix metalloproteinase-3 production in human articular chondrocytes
Furumatsu, Takayuki
Matsumoto-Ogawa, Emi
Tanaka, Takaaki
Lu, Zhichao
Ozaki, Toshifumi
CONNECTIVE TISSUE RESEARCH, 2014, 55 (02) : 89 - 95

← 1 2 3 4 5 →