From the aerial parts of Zygophyllum simplex a new glycoside was isolated and identified as 6''-(2-E-butenoyl) isorhamnetin-3-O-glucoside in addition to the known compounds: isorhamnetin, isorhamnetin 3-O-glucoside, kaempferol 3-O-rutinoside, sitosterol glucoside and quinovic acid 3-alpha-L-rhamnoside. The structures of the isolated compounds were elucidated by spectral analysis.