CLONING THE DAPA DAPB CLUSTER OF THE LYSINE-SECRETING BACTERIUM CORYNEBACTERIUM-GLUTAMICUM

被引:87
作者
CREMER, J [1 ]
EGGELING, L [1 ]
SAHM, H [1 ]
机构
[1] FORSCHUNGSZENTRUM JULICH, INST BIOTECHNOL, PF 1913, W-5170 JULICH 1, GERMANY
来源
MOLECULAR AND GENERAL GENETICS | 1990年 / 220卷 / 03期
关键词
Cluster formation; Corynebacterium glutamicum; Dihydrodipicolinate reductase; Dihydrodipicolinate synthase; Lysine biosynthesis;
D O I
10.1007/BF00391757
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The genes encoding the two successive enzymes of the lysine biosynthetic pathway, dihydrodipicolinate synthase (dapA) and dihydrodipicolinate reductase (dapB), have been isolated from Corynebacterium glutamicum by heterologous complementation of Escherichia coli mutants. The two genes reside on a single 3.8-kb chromosomal fragment. They were subcloned as non overlapping fragments on an E. coli/C. glutamicum shuttle vector and introduced into C. glutamicum. This resulted in overexpression of both enzyme activities which was irrespective of the orientation of the inserts and comparable to that obtained with the large 3.8-kb fragment. Therefore, both genes are located in close proximity to each other on the C. glutamicum chromosome, but are apparently independently transcribed. © 1990 Springer-Verlag.
引用
收藏
页码:478 / 480
页数:3
相关论文
共 12 条
[1]   MUTANTS OF ESCHERICHIA-COLI WITH A GROWTH REQUIREMENT FOR EITHER LYSINE OR PYRIDOXINE [J].
BUKHARI, AI ;
TAYLOR, AL .
JOURNAL OF BACTERIOLOGY, 1971, 105 (03) :988-&
[2]  
CREMER J, 1988, J GEN MICROBIOL, V134, P3221
[3]  
Hanahan D., 1985, DNA CLONING PRACTICA, P109
[4]   CLONING AND SEQUENCING OF THE MESO-DIAMINOPIMELATE-D-DEHYDROGENASE (DDH) GENE OF CORYNEBACTERIUM-GLUTAMICUM [J].
ISHINO, S ;
MIZUKAMI, T ;
YAMAGUCHI, K ;
KATSUMATA, R ;
ARAKI, K .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1988, 52 (11) :2903-2909
[5]   PROTOPLAST TRANSFORMATION OF GLUTAMATE-PRODUCING BACTERIA WITH PLASMID DNA [J].
KATSUMATA, R ;
OZAKI, A ;
OKA, T ;
FURUYA, A .
JOURNAL OF BACTERIOLOGY, 1984, 159 (01) :306-311
[6]   INFLUENCE OF INCREASED ASPARTATE AVAILABILITY ON LYSINE FORMATION BY A RECOMBINANT STRAIN OF CORYNEBACTERIUM-GLUTAMICUM AND UTILIZATION OF FUMARATE [J].
MENKEL, E ;
THIERBACH, G ;
EGGELING, L ;
SAHM, H .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (03) :684-688
[7]  
Patte J.-C., 1983, AMINO ACIDS BIOSYNTH, P213
[8]   MOLECULAR-CLONING, CHARACTERIZATION, AND CHROMOSOMAL LOCALIZATION OF DAPF, THE ESCHERICHIA-COLI GENE FOR DIAMINOPIMELATE EPIMERASE [J].
RICHAUD, C ;
HIGGINS, W ;
MENGINLECREULX, D ;
STRAGIER, P .
JOURNAL OF BACTERIOLOGY, 1987, 169 (04) :1454-1459
[9]  
RICHAUD F, 1981, CR ACAD SCI III-VIE, V293, P507
[10]   CHROMOSOMAL LOCATION AND NUCLEOTIDE-SEQUENCE OF THE ESCHERICHIA-COLI-DAPA GENE [J].
RICHAUD, F ;
RICHAUD, C ;
RATET, P ;
PATTE, JC .
JOURNAL OF BACTERIOLOGY, 1986, 166 (01) :297-300