THE MTR LOCUS IS A 2-GENE OPERON REQUIRED FOR TRANSCRIPTION ATTENUATION IN THE TRP OPERON OF BACILLUS-SUBTILIS

被引：81

作者：

GOLLNICK, P ^{[1
]}

ISHINO, S ^{[1
]}

KURODA, MI ^{[1
]}

HENNER, DJ ^{[1
]}

YANOFSKY, C ^{[1
]}

机构：

[1] GENENTECH INC,DEPT CELL GENET,SAN FRANCISCO,CA 94080

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 22期

关键词：

In vivo regulation; Regulatory mutants; RNA binding protein; Transcription regulator;

D O I：

10.1073/pnas.87.22.8726

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have cloned and characterized the mtr operon of Bacillus subtilis. This operon encodes a presumed RNA-binding regulatory protein that is required for attenuation control of the trp operon. We have shown that the mtr operon consists of two structural genes, mtrA and mtrB, predicted to encode 22-kDa and S-kDa polypeptides, respectively. MtrB shows homology with RegA, an RNA-binding regulatory protein of bacteriophage T4. The lesions in several mtr mutants were localized to mtrB or the putative mtr promoter. Several mtrB alleles were dominant to mtr+, suggesting that the regulatory factor is a multimeric protein. The in vivo action of the mtrA and mtrB gene products was analyzed in an E. coli strain containing a trpE-lacZ gene fusion under control of the B. subtilis trp promoter/attenuator region. Both MtrA and MtrB were necessary for regulation of β-galactosidase production.

引用

页码：8726 / 8730

页数：5

共 31 条

[1] CLONING, NUCLEOTIDE-SEQUENCE, AND OVEREXPRESSION OF THE BACTERIOPHAGE-T4 REGA GENE [J].