SPECIES-SPECIFIC EXPRESSION OF CYTOCHROME-C-OXIDASE SUBUNIT-III MESSENGER-RNA

被引：2
作者：
HOOD, DA [1 ]
机构：
[1] UNIV CONSTANCE, FAC BIOL, W-7750 CONSTANCE, GERMANY
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 97卷 / 02期
关键词：
D O I：
10.1016/0305-0491(90)90277-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. 1. A cDNA probe encoding cytochrome c oxidase subunit III cloned from rat liver mitochondria was used to quantify mRNA levels in rat, mouse and rabbit tissues. This wasvcompared to its phenotypic expression, using enzyme activity. 2. 2. Enzyme activities were highest in mouse, intermediate in rat, and lowest in rabbit tissues. 3. 3. Subunit III mRNA levels were easily quantified in rat, but could not be accurately measured in rabbit or mouse tissues despite high cytochrome c oxidase activities. 4. 4. Significant subunit III sequence divergence must exist among these species. Caution should be exercised in quantifying the expression of this mitochondrial gene. © 1990.
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页码：257 / 260
页数：4
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