CHEMICALLY-INDUCED VIRAL RESISTANCE IN SINGLE - PREIMPLANTATION BOVINE EMBRYOS

被引：1
作者：
THOMSON, MS
BIRD, RC
STRINGFELLOW, DA
ROSSI, CR
LAUERMAN, LH
机构：
[1] AUBURN UNIV,COLL VET MED,DEPT PATHOBIOL,AUBURN,AL 36849
[2] ALABAMA DEPT AGR & IND,CHARLES S ROBERTS VET DIAGNOST LAB,AUBURN,AL 36831
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 01期
关键词：
D O I：
10.1016/S0006-291X(05)81192-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Viral mRNA encoding vesicular stomatitis virus glycoprotein sequences was detected and quantitated using a DNA-hybridization dot-blot technique. This assay was employed to determine if the synthetic double-stranded polynucleotide complex of polyriboinosinic-polyribocytidylic acid would elicit viral resistance in vitro in single day 9 bovine embryos. The levels of viral mRNA were assayed in 4 groups of bovine embryos: unexposed, virus-exposed, polynucleotide-treated, and virus plus polynucleotide treated. Reduced quantities of viral mRNA in single polynucleotide treated embryos demonstrated that resistance to viral infection was induced in day 9 bovine embryos. © 1991 Academic Press, Inc.
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页码：10 / 14
页数：5
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