THE ARCB GENE OF ESCHERICHIA-COLI ENCODES A SENSOR-REGULATOR PROTEIN FOR ANAEROBIC REPRESSION OF THE ARC MODULON

被引：166
作者：
IUCHI, S
FUJIWARA, T
LIN, ECC
机构：
[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,200 LONGWOOD AVE,BOSTON,MA 02115
[2] HARVARD UNIV,SCH PUBL HLTH,DEPT CANC BIOL,BOSTON,MA 02115
来源：
MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 05期
关键词：
D O I：
10.1111/j.1365-2958.1990.tb00642.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The arcA (dye) and arcB genes of Escherichia coli are responsible for anaerobic repression of target operons and regulons of aerobic function (the arc modulon). The amino acid sequence of ArcA (Dye) indicated that it is the regulator protein of a two‐component control system. Here we show that ArcB is a membrane sensor protein on the basis of its deduced amino acid sequence (778 residues), hydropathicity profile, and cellular distribution. On the carboxyl end of the ArcB sequence there is an additional domain showing homology with conserved regions of regulator proteins. Deletion into this domain destroyed ArcB function. ArcB conserved a histidine residue for autophosphorylation of the sensor proteins, and aspartic residues important for the regulator proteins. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：715 / 727
页数：13
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