EFFECTS OF HYPERINSULINEMIA ON MUSCLE-FIBER COMPOSITION AND CAPILLARIZATION IN RATS

被引:62
作者
HOLMANG, A [1 ]
BRZEZINSKA, Z [1 ]
BJORNTORP, P [1 ]
机构
[1] UNIV GOTEBORG,SAHLGRENS HOSP,DEPT HEART & LUNG DIS,S-41345 GOTHENBURG,SWEDEN
关键词
D O I
10.2337/diabetes.42.7.1073
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Statistical studies repeatedly have shown an association between systemic insulin resistance and a preponderance of highly glycolytic, relatively insulin-insensitive muscle fibers as well as a low density of muscle capillaries. The nature of the relationship between these observations is, however, not clear. Female rats were made hyperinsulinemic for 7 days by implantation of osmotic minipumps. Elevated adrenergic activity and secretion of glucocorticoids were controlled by another minipump with propranolol and adrenalectomy was controlled with glucocorticoid substitution. This resulted in hyperinsulinemia and moderate hypoglycemia, the latter probably counteracted by overeating and increased glucagon secretion, as indicated by increased body weight and lower liver glycogen contents, respectively. Systemic insulin sensitivity was increased and measured with a hyperinsulinemic-euglycemic clamp technique. This was paralleled by an elevated glucose utilization estimated as uptake of 2-deoxyglucose in parametrial, retroperitoneal, and inguinal adipose tissues and the soleus and extensor digitorum longus muscles. Glycogen synthesis was also elevated in the soleus muscle. Muscle fiber composition changed with hyperinsulinemia and elevated 2-deoxyglucose uptake toward more fast-twitch, type II, particularly type IIb fibers, whereas the proportion of slow-twitch, type I fibers, diminished. Capillary density was elevated per unit muscle surface area as well as per muscle fiber. This was paralleled by increased insulin sensitivity systemically and in muscles. These results suggest that muscle fiber composition alterations may be a consequence rather than a cause of hyperinsulinemia and that capillarization rather than fiber composition is of importance for insulin sensitivity in muscle.
引用
收藏
页码:1073 / 1081
页数:9
相关论文
共 45 条
[1]   INSULIN, INSULIN-LIKE GROWTH-FACTORS, AND VASCULAR ENDOTHELIUM [J].
BAR, RS ;
BOES, M ;
DAKE, BL ;
BOOTH, BA ;
HENLEY, SA ;
SANDRA, A .
AMERICAN JOURNAL OF MEDICINE, 1988, 85 (5A) :59-70
[2]  
BIA MJ, 1977, AM J PHYSIOL, V233, pF509
[3]   INSULIN BINDING AND GLUCOSE-UPTAKE DIFFERENCES IN RODENT SKELETAL-MUSCLES [J].
BONEN, A ;
TAN, MH ;
WATSONWRIGHT, WM .
DIABETES, 1981, 30 (08) :702-704
[4]   INSULIN RESISTANCE IN SOLEUS MUSCLE FROM OBESE ZUCKER RATS - INVOLVEMENT OF SEVERAL DEFECTIVE SITES [J].
CRETTAZ, M ;
PRENTKI, M ;
ZANINETTI, D ;
JEANRENAUD, B .
BIOCHEMICAL JOURNAL, 1980, 186 (02) :525-534
[5]   METABOLIC CONSEQUENCES OF HYPERINSULINEMIA IMPOSED ON NORMAL RATS ON GLUCOSE HANDLING BY WHITE ADIPOSE-TISSUE, MUSCLES AND LIVER [J].
CUSIN, I ;
TERRETTAZ, J ;
ROHNERJEANRENAUD, F ;
JEANRENAUD, B .
BIOCHEMICAL JOURNAL, 1990, 267 (01) :99-103
[6]   THE INFLUENCE OF PREDNISONE ON THE MUSCLE MORPHOLOGY AND MUSCLE ENZYMES IN PATIENTS WITH RHEUMATOID-ARTHRITIS [J].
DANNESKIOLDSAMSOE, B ;
GRIMBY, G .
CLINICAL SCIENCE, 1986, 71 (06) :693-701
[7]   A METHOD TO QUANTIFY GLUCOSE-UTILIZATION INVIVO IN SKELETAL-MUSCLE AND WHITE ADIPOSE-TISSUE OF THE ANESTHETIZED RAT [J].
FERRE, P ;
LETURQUE, A ;
BURNOL, AF ;
PENICAUD, L ;
GIRARD, J .
BIOCHEMICAL JOURNAL, 1985, 228 (01) :103-110
[8]   INSULIN INDUCES PROGRESSIVE INSULIN RESISTANCE IN CULTURED RAT ADIPOCYTES - SEQUENTIAL EFFECTS AT RECEPTOR AND MULTIPLE POSTRECEPTOR SITES [J].
GARVEY, WT ;
OLEFSKY, JM ;
MARSHALL, S .
DIABETES, 1986, 35 (03) :258-267
[9]  
GERGELY J, 1983, HDB PHYSL 10, P257
[10]   FACTORS INVOLVED IN THE CONTROL OF PROLIFERATION OF BOVINE CORNEAL ENDOTHELIAL-CELLS MAINTAINED IN SERUM-FREE MEDIUM [J].
GIGUERE, L ;
CHENG, J ;
GOSPODAROWICZ, D .
JOURNAL OF CELLULAR PHYSIOLOGY, 1982, 110 (01) :72-80