LARGE-SCALE PURIFICATION AND IMMUNOLOGICAL CHARACTERIZATION OF HUMAN PLACENTAL NERVE GROWTH-FACTOR

被引：9

作者：

BIGON, E ^{[1
]}

SORANZO, C ^{[1
]}

MINOZZI, C ^{[1
]}

SKAPER, SD ^{[1
]}

CALLEGARO, L ^{[1
]}

机构：

[1] FIDIA RES LABS,FIDIA RES LABS,ABANO TERME,ITALY

来源：

NEUROCHEMICAL RESEARCH | 1990年 / 15卷 / 12期

关键词：

NERVE GROWTH FACTOR; HUMAN PLACENTA; IMMUNOLOGICAL CROSS-REACTIVITY; MONOCLONAL-POLYCLONAL ANTIBODIES;

D O I：

10.1007/BF01208580

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nerve growth factor (NGF) is a protein which plays a critical role in the development and survival of not only peripheral neurons, but possibly also cholinergic brain neurons. The present study describes a procedure for large scale isolation of human NGF of placental origin, and its immunological characterization. A protein species of approximately 26 kDa was obtained, which cross-reacted with antibodies to mouse NGF. Polyclonal and monoclonal anti-mouse NGF antibodies appeared to recognize different bands within this human NGF preparation. Although these polyclonal antibodies recognized both the dimeric and monomeric forms of mouse NGF, the monoclonal antibody recognized only a band corresponding to the dimeric form of mouse NGF.

引用

页码：1197 / 1202

页数：6

共 24 条

[1] THE CHOLINERGIC HYPOTHESIS OF GERIATRIC MEMORY DYSFUNCTION [J].