ASCORBIC ACID EFFECTS ON SEROTONIN FLUORESCENCE

被引：7

作者：

THOMPSON, JH

SPEZIA, CA

ANGULO, M

机构：

[1] Department of Pharmacology, Experimental Therapeutics, UCLA School of Medicine, Los Angeles

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 31卷 / 1-3期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(69)90272-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ascorbic acid successfully protects serotonin from catalytic destruction by iron during fluorometric detection but amine stability is accompanied by diminished fluorescence. The reduction of fluorescence has been found to result from absorption of 295 mμ wavelength activation light and from quenching of serotonin fluorescence by ascorbic acid. Ascorbic acid in aqueous 3.5 N HCl was found to have molar absorptivity (ε) of 4.4 ± 0.1 (n = 6) and a quenching constant (kq) of 11.0 ± 0.2 (n = 8). © 1969.

引用

页码：321 / &

共 7 条

[1] AN IMPROVED METHOD FOR FLUORIMETRIC DETERMINATION OF 5-HYDROXYTRYPTAMINE IN TISSUES [J].