CHARACTERIZATION OF PAPAYA PEPTIDASE-A AS A CYSTEINE PROTEINASE OF CARICA-PAPAYA L WITH ACTIVE-CENTER PROPERTIES THAT DIFFER FROM THOSE OF PAPAIN BY USING 2,2'-DIPYRIDYL DISULFIDE AND 4-CHLORO-7-NITROBENZOFURAZAN AS REACTIVITY PROBES - USE OF 2-PROTONIC-STATE ELECTROPHILES IN THE IDENTIFICATION OF CATALYTIC-SITE THIOL-GROUPS

被引：28
作者：
BAINES, BS [1 ]
BROCKLEHURST, K [1 ]
机构：
[1] UNIV LONDON,ST BARTHOLOMEWS HOSP,COLL MED,DEPT BIOCHEM & CHEM,LONDON EC1M 6BQ,ENGLAND
来源：
BIOCHEMICAL JOURNAL | 1982年 / 205卷 / 01期
关键词：
D O I：
10.1042/bj2050205
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
引用
收藏
页码：205 / 211
页数：7
相关论文
共 25 条
[1]   MECHANISM OF ACTION OF PAPAIN WITH A SPECIFIC ANILIDE SUBSTRATE [J].
ANGELIDES, KJ ;
FINK, AL .
BIOCHEMISTRY, 1979, 18 (11) :2355-2363
[2]   CRYOENZYMOLOGY OF PAPAIN - REACTION-MECHANISM WITH AN ESTER SUBSTRATE [J].
ANGELIDES, KJ ;
FINK, AL .
BIOCHEMISTRY, 1978, 17 (13) :2659-2668
[3]   MECHANISM OF THIOL PROTEASE CATALYSIS - DETECTION AND STABILIZATION OF A TETRAHEDRAL INTERMEDIATE IN PAPAIN CATALYSIS [J].
ANGELIDES, KJ ;
FINK, AL .
BIOCHEMISTRY, 1979, 18 (11) :2363-2369
[4]  
Baines BS, 1982, J PROTEIN CHEM, V1, P119
[5]  
BOUDART R, 1970, Patent No. 1196760
[6]  
BOUDART R, 1969, Patent No. 723163
[7]   DIFFERENCES IN THE INTERACTIONS OF THE CATALYTIC GROUPS OF THE ACTIVE-CENTERS OF ACTINIDIN AND PAPAIN - RAPID PURIFICATION OF FULLY ACTIVE ACTINIDIN BY COVALENT CHROMATOGRAPHY AND CHARACTERIZATION OF ITS ACTIVE-CENTER BY USE OF 2-PROTONIC-STATE REACTIVITY PROBES [J].
BROCKLEHURST, K ;
BAINES, BS ;
MALTHOUSE, JPG .
BIOCHEMICAL JOURNAL, 1981, 197 (03) :739-746
[8]   EVIDENCE THAT THE LACK OF HIGH CATALYTIC ACTIVITY OF THIOLSUBTILISIN TOWARDS SPECIFIC SUBSTRATES MAY BE DUE TO AN INAPPROPRIATELY LOCATED PROTON-DISTRIBUTION SYSTEM - DEMONSTRATION OF HIGHLY NUCLEOPHILIC CHARACTER OF THE THIOL-GROUP OF THIOSUBTILISIN IN THE CATALYTICALLY RELEVANT IONIZATION STATE OF THE ACTIVE-CENTER BY USE OF A 2-PROTONIC-STATE REACTIVITY PROBECL [J].
BROCKLEHURST, K ;
MALTHOUSE, JPG .
BIOCHEMICAL JOURNAL, 1981, 193 (03) :819-823
[9]  
BROCKLEHURST K, 1982, METHOD ENZYMOL, V87, P427
[10]   EVIDENCE THAT BINDING TO THE S-2-SUBSITE OF PAPAIN MAY BE COUPLED WITH CATALYTICALLY RELEVANT STRUCTURAL-CHANGE INVOLVING THE CYSTEINE-25-HISTIDINE-159 DIAD - KINETICS OF THE REACTION OF PAPAIN WITH A 2-PROTONIC-STATE REACTIVITY PROBE CONTAINING A HYDROPHOBIC SIDE-CHAIN [J].
BROCKLEHURST, K ;
MALTHOUSE, JPG ;
SHIPTON, M .
BIOCHEMICAL JOURNAL, 1979, 183 (02) :223-231
123