AUTOCRINE BIOLOGICAL EFFECTS OF GLYCOSYL INOSITOL PHOSPHATE PRODUCED BY RECONSTITUTED PIG THYROID-FOLLICLES - ROLE OF PERTUSSIS TOXIN SENSITIVE G-PROTEINS

被引：25
作者：
MARTINY, L
DELEMER, B
PETITFRERE, E
LAMBERT, B
JACQUEMIN, C
HAYE, B
机构：
[1] HOP BICETRE,INSERM,U96,F-94276 LE KREMLIN BICETR,FRANCE
[2] FAC SCI REIMS,F-51062 REIMS,FRANCE
来源：
CELLULAR SIGNALLING | 1992年 / 4卷 / 02期
关键词：
THYROCYTES; GLYCOSYL INOSITOL PHOSPHATE; PERTUSSIS TOXIN; CAMP; IODIDE UPTAKE; IODIDE ORGANIFICATION; THYROTROPIN;
D O I：
10.1016/0898-6568(92)90085-M
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We examine the autocrine activity of glycosyl inositol phosphate (InP-gly) on thyroid metabolism. The cAMP accumulation promoted by thyrotropin (TSH) or forskolin was modulated by InP-gly, stimulated by the lowest tested concentration (10(-8) M) and progressively inhibited by higher concentrations. Iodide uptake and iodine organification were decreased in a concentration-dependent manner by InP-gly alone, or in the presence of TSH. The IAP component of pertussis toxin blocked the inhibitory action of InP-gly on cAMP accumulation by reconstituted thyroid follicles (RTF), suggesting the participation of G(i) protein. But the same treatment with IAP was without effect on iodine metabolism, suggesting that there is a second target for InP-gly, more distal than G(i) protein, or coupled to another G rotein which is insensitive to the toxin.
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收藏
页码：219 / 229
页数：11
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