BICARBONATE MEASUREMENTS IN RAT-LIVER, BRAIN, HEART, AND SKELETAL-MUSCLE

被引：9
作者：
KING, MT [1 ]
GAMBLE, JL [1 ]
VEECH, RL [1 ]
机构：
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PHYSIOL,BALTIMORE,MD 21218
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期
关键词：
D O I：
10.1016/0003-2697(79)90202-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method is described for measuring total CO2 and HCO3 - in tissues rapidly frozen in liquid nitrogen. The method is a modification of the procedure of D. D. Van Slyke and J. M. Neill (1924, J. Biol. Chem. 61, 523-573) for use with freeze-clamped tissue where anoxic changes have not occurred. The HCO3 - content exclusive of tissue CO2 in fed rats was found to be: liver, 19.4 ± 1.0; brain, 20.2 ± 0.9; thigh, 16.2 ± 0.8; and heart, 15.4 ± 1.4 μmol/g. © 1979.
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页码：183 / 187
页数：5
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