A NON-SPECIFIC ADENINE NUCLEOTIDE DEAMINASE FROM DESULFOVIBRIO DESULFURICANS

被引：14
作者：
YATES, MG
机构：
[1] A.R.C. Unit of Nitrogen Fixation, University of Sussex, Brighton,, Sussex Sussex
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 171卷 / 02期
关键词：
D O I：
10.1016/0005-2744(69)90163-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. 1. A non-specific adenine nucleotide deaminase, which is equally active with AMP, ADP or AtP, was extracted from Desulfovibrio desulfuricans, strain Berre S, and purified approx. 800-fold. 2. 2. Enzyme preparations contained 4% of nucleic acids, which stabilised the activity during storage. 3. 3. The enzyme was competitively inhibited by various substances with the following values for Ki: urea (410 mM), p-mercuribenzoate (11.8 μM), HgCl2 (5.3 μM), CuSO4 (67 μM) and the anions, β,β dimethyl glutarate (3.2 mM), citrate (6.0 mM), succinate (18 mM), maleate (22 mM), phosphate (83 mM), cacodylate (134 mM) and Cl-. 4. 4. Low concentrations of F- and CN- caused non-competitive inhibition. © 1969.
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页码：299 / &
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