IMMOBILIZATION OF MERCURIC REDUCTASE FROM A PSEUDOMONAS-PUTIDA STRAIN ON DIFFERENT ACTIVATED CARRIERS

被引：8

作者：

ANSPACH, FB ^{[1
]}

HUCKEL, M ^{[1
]}

BRUNKE, M ^{[1
]}

SCHUTTE, H ^{[1
]}

DECKWER, WD ^{[1
]}

机构：

[1] TFH BERLIN,W-1000 BERLIN 65,GERMANY

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 44卷 / 02期

关键词：

MERCURIC REDUCTASE; IMMOBILIZATION; EUPERGIT C; CYANOGEN BROMIDE-; TRESYL CHLORIDE-; CDI-; OXIRANE-ACTIVATED SEPHAROSE 4B;

D O I：

10.1007/BF02921651

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mercuric reductase was isolated from Pseudomonas putida KT2442::mer-73 and immobilized on chromatographic carriers activated by various methods. The immobilization methods for covalent coupling were compared with regard to preservation of enzymatic activity and coupling yields. Highest yields were obtained with carriers bearing the most reactive functional groups. Best results were achieved with tresyl chloride-activated carriers. The optimum binding conditions were found at pH 8. Application of the immobilized mercuric reductase for continuous treatment of Hg(II)-containing water was examined in a fixed bed reactor. Space-time yields up to 510 nmol/min.ml were attained. The kinetics of immobilized enzyme systems were not diffusion-controlled

引用

页码：135 / 150

页数：16

共 22 条

[1] KINETIC-STUDIES OF SEPHAROSE-IMMOBILIZED AND CH-SEPHAROSE-IMMOBILIZED DIHYDROFOLATE-REDUCTASE [J].