COMPARISON OF MOUSE AND HUMAN HOX-4 COMPLEXES DEFINES CONSERVED SEQUENCES INVOLVED IN THE REGULATION OF HOX-4.4

被引：62
作者：
RENUCCI, A
ZAPPAVIGNA, V
ZAKANY, J
IZPISUABELMONTE, JC
BURKI, K
DUBOULE, D
机构：
[1] EUROPEAN MOLEC BIOL LAB,MEYERHOFSTR 1,W-6900 HEIDELBERG,GERMANY
[2] SANDOZ PHARMA AG,PRECLIN RES,CH-4002 BASEL,SWITZERLAND
来源：
EMBO JOURNAL | 1992年 / 11卷 / 04期
关键词：
DEVELOPMENT; HOMEOBOX; HOX-4; COMPLEX; TRANSGENESIS;
D O I：
10.1002/j.1460-2075.1992.tb05190.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned and sequenced, in both mouse and human, regions of the HOX-4 complex which contain two Abd-B like genes, Hox-4.4 and Hox-4.5 (HOX4C and HOX4D in human, respectively). The high degree of conservation between the homeoprotein sequences extends to non-coding areas, which suggests that the mechanisms of regulation have been conserved. We show that the Hox-4.5/Hox-4.4 intergenic region can be broadly subdivided into three domains based on DNA conservation between rodents and primates. The presence of all these domains in association with sequences located 3' to the transcription termination site are required to mimic the spatial regulation of Hox4.4 in transgenic mouse embryos. Several highly conserved short sequences located in this region were studied in gel retardation assays for their binding to potential regulatory factors. One such factor is detected in embryonal carcinoma cells but absent from other differentiated cell lines. This specific binding activity is down regulated upon retinoic acid treatment.
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收藏
页码：1459 / 1468
页数：10
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