STEREOCHEMICAL ANALYSIS OF BETAMETHASONE AND DEXAMETHASONE BY DERIVATIZATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：19

作者：

CHEN, SH ^{[1
]}

WU, SM ^{[1
]}

WU, HL ^{[1
]}

机构：

[1] KAOHSIUNG MED COLL,GRAD INST PHARMACEUT SCI,KAOHSIUNG 807,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 595卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)85163-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and economical high-performance liquid chromatographic method has been developed for the simultaneous determination of betamethasone and dexamethasone. The method is based on the derivatization of the structural epimers of betamethasone and dexamethasone with a homochiral reagent, N-carbobenzoxy-L-phenylalanine. The derivatives obtained were easily recognized by a non-chiral silica column with n-hexane dichloromethane-isopropanol (100:100:4, v/v/v) as a mobile phase and a good separation was obtained for quantitation. The method was satisfactorily applied to the determination of betamethasone and dexamethasone in tablets.

引用

页码：203 / 208

页数：6

共 14 条

[1]

BUNCH EA, 1987, J ASSOC OFF ANA CHEM, V70, P967

[2] HIGH-FIELD H-1 AND C-13 NMR-SPECTROSCOPY OF SOME CORTICOSTEROIDS AND RELATED-COMPOUNDS [J].

DUDDECK, H ;

ROSENBAUM, D ;

HANI, M ;

ELGAMAL, A ;

FAYEZ, MBE .

MAGNETIC RESONANCE IN CHEMISTRY, 1986, 24 (11) :999-1003

[3] APPLICATIONS OF CHROMATOGRAPHIC AND SPECTROMETRIC TECHNIQUES TO THE STUDY OF DEXAMETHASONE RELATED-COMPOUNDS [J].

GAIGNAGE, P ;

LOGNAY, G ;

MARLIER, M ;

SEVERIN, M ;

DREZE, P .

CHROMATOGRAPHIA, 1989, 28 (11-12) :623-630

[4]

KALANT H, 1989, PRINCIPLES MED PHARM, P479

[5]

LAMIABLE D, 1986, J CHROMATOGR, V378, P486

[6]

LAMIABLE D, 1988, J CHROMATOGR, V2434, P315

[7] DETERMINATION OF BETAMETHASONE 17-BENZOATE IN LIPOPHYLIC VEHICLES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

MARON, N ;

CRISTI, EA ;

RAMOS, AA .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1988, 77 (07) :638-639

[8] DETERMINATION OF DEXAMETHASONE IN BOVINE-TISSUES BY COUPLED-COLUMN NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

MCLAUGHLIN, LG ;

HENION, JD .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 529 (01) :1-19

[9]

NG LL, 1987, J ASSOC OFF ANA CHEM, V70, P829

[10]

NG LL, 1988, J ASSOC OFF ANA CHEM, V71, P534

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