RAPID THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE DETECTION OF URINARY METHYLMALONIC ACID

被引：14

作者：

AURAYBLAIS, C ^{[1
]}

GIGUERE, R ^{[1
]}

PARADIS, D ^{[1
]}

LEMIEUX, B ^{[1
]}

机构：

[1] UNIV SHERBROOKE, MED CTR,DEPT PEDIAT,PEDIAT RES UNIT, QUEBEC GENET SCREENING PROGRAM, SHERBROOKE J1H 5N4, QUEBEC, CANADA

来源：

CLINICAL BIOCHEMISTRY | 1979年 / 12卷 / 02期

关键词：

D O I：

10.1016/S0009-9120(79)80003-X

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

1.Simple and rapid thin-layer and micro-thin-layer chromatography techniques are described for the detection of methylmalonic acid in urine.2.The separation of methylmalonic acid from a crude urine sample is performed by thin-layer chromatography with a mixture of silica gel-cellulose and a butanol - acetic acid - water solvent system. The methylmalonic acid spot is visualized with tetrazotized o-dianisidine.3.This system has been successfully developed for a urinary screening programme; it was shown as simple, convenient and rapid, eliminating false-positives and allowing the detection of even traces of methylamalonic acid. © 1979 Canadian Society of Clinical Chemists.

引用

页码：43 / 45

页数：3

共 9 条

[1] RAPID DETECTION OF METHYLMALONIC ACID IN URINE - A SENSITIVE INDEX OF VITAMIN B12 DEFICIENCY [J].