EVIDENCE FOR THE COEXPRESSION OF OXYTOCIN AND VASOPRESSIN MESSENGER RIBONUCLEIC-ACIDS IN MAGNOCELLULAR NEUROSECRETORY-CELLS - SIMULTANEOUS DEMONSTRATION OF 2 NEUROHYPOPHYSIN MESSENGER RIBONUCLEIC-ACIDS BY HYBRIDIZATION HISTOCHEMISTRY

被引：89

作者：

KIYAMA, H ^{[1
]}

EMSON, PC ^{[1
]}

机构：

[1] AFRC,INST ANIM PHYSIOL & GENET RES,MRC GRP,CAMBRIDGE CB2 4AT,ENGLAND

来源：

JOURNAL OF NEUROENDOCRINOLOGY | 1990年 / 2卷 / 03期

关键词：

messenger ribonucleic acid ‘co‐expression; oxytocin; salt loading; vasopressin;

D O I：

10.1111/j.1365-2826.1990.tb00401.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A novel enzyme‐labelled oligonucleotide probe specific for oxytocin messenger ribonucleic acid (mRNA) and a radiolabelled oligonucleotide probe specific for vasopressin mRNA were used together to visualize both oxytocin and vasopressin mRNA's in hypothalamus sections from salt loaded (2% saline) rats. The results demonstrate that the majority of magnocellular neurons contain only oxytocin or vasopressin mRNA, however a small number of neurons, 1% to 2%, contained both oxytocin and vasopressin transcripts. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：257 / &

共 7 条

[1] SIMULTANEOUS VISUALIZATION OF VASOPRESSIN AND OXYTOCIN MESSENGER RNA-CONTAINING NEURONS IN THE HYPOTHALAMUS USING NON-RADIOACTIVE INSITU HYBRIDIZATION HISTOCHEMISTRY
ICHIMIYA, Y
EMSON, PC
CHRISTODOULOU, C
GAIT, MJ
RUTH, JL
[J]. JOURNAL OF NEUROENDOCRINOLOGY, 1989, 1 (02) : 73 - 75
[2] PREPARATION OF OLIGODEOXYNUCLEOTIDE ALKALINE-PHOSPHATASE CONJUGATES AND THEIR USE AS HYBRIDIZATION PROBES
JABLONSKI, E
MOOMAW, EW
TULLIS, RH
RUTH, JL
[J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (15) : 6115 - 6128
[3] SENSITIVE NONRADIOISOTOPIC INSITU HYBRIDIZATION HISTOCHEMISTRY - DEMONSTRATION OF TYROSINE-HYDROXYLASE GENE-EXPRESSION IN RAT-BRAIN AND ADRENAL
KIYAMA, H
EMSON, PC
RUTH, J
MORGAN, C
[J]. MOLECULAR BRAIN RESEARCH, 1990, 7 (03): : 213 - 219
[4] ELECTROPHYSIOLOGICAL EVIDENCE FOR ACTIVATION OF SUPRAOPTIC NEURONS DURING RELEASE OF OXYTOCIN
LINCOLN, DW
WAKERLEY, JB
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1974, 242 (02): : 533 - 554
[5] EXPRESSION OF THE VASOPRESSIN AND OXYTOCIN GENES IN RATS OCCURS IN MUTUALLY EXCLUSIVE SETS OF HYPOTHALAMIC NEURONS
MOHR, E
BAHNSEN, U
KIESSLING, C
RICHTER, D
[J]. FEBS LETTERS, 1988, 242 (01) : 144 - 148
[6] RUTH JL, 1985, DNA-J MOLEC CELL BIO, V4, P93
[7] UHL GR, 1986, IN SITU HYBRIDISATIO

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