PROGESTERONE BIOTRANSFORMATION BY HUMAN P4503A4 IN YEAST

被引：7

作者：

MEHMOOD, Z ^{[1
]}

KELLY, DE ^{[1
]}

KELLY, SL ^{[1
]}

机构：

[1] UNIV SHEFFIELD, KREBS INST BIOMOLEC RES, DEPT MOLEC BIOL & BIOTECHNOL, SHEFFIELD S10 2UH, S YORKSHIRE, ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 01期

关键词：

D O I：

10.1007/BF00134201

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The biotransformation of progesterone by human P4503A4 expressed in Saccharomyces cerevisiae was studied. Cytochrome P450 was produce to a specific content of 175+/-15 pmol/mg microsomal protein. Cells and microsomal fractions containing P4503A4 efficiently converted substrate to 6 beta- and 16 alpha-hydroxy progesterone, but this was not observed for control transformants.

引用

页码：83 / 88

页数：6

共 11 条

[1] CATALYTIC ACTIVITIES OF HUMAN LIVER CYTOCHROME-P-450-IIIA4 EXPRESSED IN SACCHAROMYCES-CEREVISIAE [J].