IDENTIFICATION OF THE METHANOCOCCUS-VOLTAE S-LAYER STRUCTURAL GENE

被引:14
作者
KONISKY, J [1 ]
LYNN, D [1 ]
HOPPERT, M [1 ]
MAYER, F [1 ]
HANEY, P [1 ]
机构
[1] UNIV GOTTINGEN,INST MIKROBIOL,W-3400 GOTTINGEN,GERMANY
关键词
D O I
10.1128/jb.176.6.1790-1792.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have established that the gene which we had previously identified as encoding the Methanococcus voltae P-type ATPase is, in fact, the structural gene for the M. voltae S-lager protein. This conclusion is based on a comparison of the N-terminal sequence of S-layer protein prepared by two independent methods with that derived from the nucleotide sequence of the cloned gene. This conclusion was further supported by immuno-cytochemical localization of the antigen directed against the antibodies used in the cloning experiments.
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页码:1790 / 1792
页数:3
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