SEQUENCE-ANALYSIS AND INTERPOSON MUTAGENESIS OF THE HUPT GENE, WHICH ENCODES A SENSOR PROTEIN INVOLVED IN REPRESSION OF HYDROGENASE SYNTHESIS IN RHODOBACTER-CAPSULATUS

被引:42
作者
ELSEN, S [1 ]
RICHAUD, P [1 ]
COLBEAU, A [1 ]
VIGNAIS, PM [1 ]
机构
[1] CEN,DEPT BIOL MOLEC & STRUCT,CNRS,BIOCHIM MICROBIENNE LAB,INSERM,U1130,F-38041 GRENOBLE,FRANCE
关键词
D O I
10.1128/JB.175.22.7404-7412.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The hupT gene, which represses hydrogenase gene expression in the purple photosynthetic bacterium Rhodobacter capsulatus, has been identified and sequenced. The nucleotide sequence of hupT and of the contiguous downstream open reading frame, hupU, is reported. The HupT protein of 456 amino acids (48,414 Da) has sequence similarity with the FixL, DctB, NtrB, and ArcB proteins and is predicted to be a soluble sensor kinase. Insertional inactivation of the hupT gene led to deregulation of transcriptional control, so that the hydrogenase structural operon hupSLC became overexpressed in cells grown anaerobically or aerobically. The HupT- mutants were complemented in trans by a plasmid containing an intact copy of the hupT gene. The hupU open reading frame, capable of encoding a protein of 84,879 Da, shared identity with [NiFe]hydrogenase subunits; the strongest similarity was observed with the periplasmic hydrogenase of Desulfovibrio baculatus.
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页码:7404 / 7412
页数:9
相关论文
共 58 条
[51]  
VIGNAIS PM, IN PRESS ARCH MICROB
[52]   CHARACTERIZATION OF RHODOPSEUDOMONAS-CAPSULATA [J].
WEAVER, PF ;
WALL, JD ;
GEST, H .
ARCHIVES OF MICROBIOLOGY, 1975, 105 (03) :207-216
[53]   THE CPX PROTEINS OF ESCHERICHIA-COLI-K12 - STRUCTURE OF THE CPXA POLYPEPTIDE AS AN INNER MEMBRANE COMPONENT [J].
WEBER, RF ;
SILVERMAN, PM .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (02) :467-478
[54]  
WILLISON JC, 1993, FEMS MICROBIOL LETT, V104, P1
[55]   THE Q-LINKER - A CLASS OF INTERDOMAIN SEQUENCES FOUND IN BACTERIAL MULTIDOMAIN REGULATORY PROTEINS [J].
WOOTTON, JC ;
DRUMMOND, MH .
PROTEIN ENGINEERING, 1989, 2 (07) :535-543
[56]   PUTATIVE NICKEL-BINDING SITES OF MICROBIAL PROTEINS [J].
WU, LF .
RESEARCH IN MICROBIOLOGY, 1992, 143 (03) :347-351
[57]   IMPROVED M13 PHAGE CLONING VECTORS AND HOST STRAINS - NUCLEOTIDE-SEQUENCES OF THE M13MP18 AND PUC19 VECTORS [J].
YANISCHPERRON, C ;
VIEIRA, J ;
MESSING, J .
GENE, 1985, 33 (01) :103-119
[58]  
[No title captured]