TANDEM MASS-SPECTROMETRIC ANALYSIS OF PEPTIDES AT THE FEMTOMOLE LEVEL

被引：14
作者：
KENNY, PTM [1 ]
ORLANDO, R [1 ]
机构：
[1] SUNTORY INST BIOORGAN RES,SHIMAMOTO,OSAKA 618,JAPAN
来源：
ANALYTICAL CHEMISTRY | 1992年 / 64卷 / 08期
关键词：
D O I：
10.1021/ac00032a022
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In the sequential analysis of peptides by fast atom bombardment (FAB) tandem mass spectrometry (MS/MS), the principal obstacle to decreasing sample quantities was determined to be the signal-to-background ratio of the ionization/desorption process. By decreasing the background ion current, continuous-flow FAB allows complete analysis (both MS and MS/MS) to be performed on 25-75% less sample than required for a conventional FABMS/MS experiment alone. The combination of CF-FAB with array detection permitted sequential analysis of several peptides (900-2000 Da) at the 900 fmol to 5.8 pmol level, without interference from the background. These levels do not produce a molecular ion species easily discernible above the background in conventional FAB.
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页码：957 / 960
页数：4
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