MOLECULAR-CLONING AND MAMMALIAN EXPRESSION OF HUMAN BETA-2-GLYCOPROTEIN-I CDNA

被引：50
作者：
KRISTENSEN, T
SCHOUSBOE, I
BOEL, E
MULVIHILL, EM
HANSEN, RR
MOLLER, KB
MOLLER, NPH
SOTTRUPJENSEN, L
机构：
[1] NOVO NORDISK AS,BIOSCI,DK-2880 BAGSVAERD,DENMARK
[2] ZYMOGENET INC,SEATTLE,WA 98105
[3] UNIV COPENHAGEN,PANUM INST,DEPT BIOCHEM C,DK-2200 COPENHAGEN,DENMARK
来源：
FEBS LETTERS | 1991年 / 289卷 / 02期
关键词：
BETA-2-GLYCOPROTEIN-I; APOLIPOPROTEIN-H; SHORT CONSENSUS REPEAT; EXPRESSION; CLONING; HOMO-SAPIENS;
D O I：
10.1016/0014-5793(91)81065-G
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human beta-2-glycoprotein (beta-2gpI) cDNA was isolated from a liver cDNA library and sequenced. The cDNA encoded a 19-residue hydrophobic signal peptide followed by the mature beta-2gpI of 326 amino acid residues. In liver and in the hepatoma cell line HepG2 there are two mRNA species of about 1.4 and 4.3 kb, respectively, hybridizing specifically with the beta-2gpI cDNA. Upon isoelectric focusing, recombinant beta-2gpI obtained from expression of beta-2gpI cDNA in baby hamster kidney cells showed the same pattern of bands as beta-2gpI isolated from plasma, and at least 5 polypeptides were visible.
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页码：183 / 186
页数：4
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