A PROTEIN KINASE-ENCODING GENE, PKT1, FROM TRICHODERMA-REESEI, HOMOLOGOUS TO THE YEAST YPK1 AND YPK2 (YKR2) GENES

被引:6
作者
MORAWETZ, R
MISCHAK, H
GOODNIGHT, J
LENDENFELD, T
MUSHINSKY, JF
KUBICEK, CP
机构
[1] TECH UNIV VIENNA, INST BIOCHEM TECHNOL & MIKROBIOL, MIKROBIELLE BIOCHEM ABT, A-1060 VIENNA, AUSTRIA
[2] NCI, GENET LAB, MOLEC GENET SECT, BETHESDA, MD 20892 USA
基金
美国国家卫生研究院;
关键词
RECOMBINANT DNA; NUCLEOTIDE SEQUENCE; POLYADENYLATION; PROTEIN KINASE C; SIGNAL TRANSDUCTION; INTRON; FILAMENTOUS FUNGI;
D O I
10.1016/0378-1119(94)90311-5
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A gene (pkt1) was isolated from the filamentous fungus Trichoderma reesei, which exhibits high homology with the yeast YPK1 and YKR2 (YKR2) genes. It contains a 2123-bp ORF that is interrupted by two introns, and it encodes a 662-amino-acid protein with a calculated M(r) of 72 820. During active growth, pkt1 is expressed as two mRNAs of 3.1 and 2.8 kb which differ in the 3' untranslated region due to the use of two different polyadenylation sites.
引用
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页码:309 / 310
页数:2
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