THE BE (2)-M17 NEUROBLASTOMA CELL-LINE SYNTHESIZES AND SECRETES CORTICOTROPIN-RELEASING FACTOR

被引:10
作者
KASCKOW, JW
PARKES, DG
OWENS, MJ
STIPETIC, MD
HAN, JH
NEMEROFF, CB
VALE, WW
机构
[1] SALK INST,CLAYTON FDN LABS PEPTIDE BIOL,SAN DIEGO,CA 92186
[2] HOWARD FLOREY INST EXPTL PHYSIOL & MED,PARKVILLE,VIC 3052,AUSTRALIA
关键词
CORTICOTROPIN-RELEASING FACTOR; RIA; NEUROBLASTOMA; HPLC; FORSKOLIN; BE (2)-M17;
D O I
10.1016/0006-8993(94)91583-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The BE (2)-M17 human neuroblastoma has previously been shown to express corticotropin-releasing factor (CRF) mRNA following retinoic acid treatment. It is demonstrated in this report that both cell extracts and cell incubation medium of retinoic acid-treated BE (2)-M17 cells were shown to contain CRF-like immunoreactivity (CRF-LI) by RIA. CRF-LI secretion and content were also dose-dependently increased by forskolin. In addition, cell extracts were applied to a C-18 Vydac column and peak CRF-LI from the collected fractions was shown to coincide in time of elution with peak immunoreactivity seen with oxidized synthetic CRF standard. Thus, in containing the CRF peptide, the BE (2)-M17 cells are useful models for further study of CRF cellular and genetic regulation.
引用
收藏
页码:159 / 162
页数:4
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