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INTRACELLULAR GALACTOSE-OXIDASE FROM A MUTANT STRAIN DEFECTIVE IN ITS SECRETION
被引：0
作者：
KITAMURA, AE
AMARAL, D
ZANCAN, GT
机构：
来源：
ARQUIVOS DE BIOLOGIA E TECNOLOGIA
|
1994年
/
37卷
/
04期
关键词：
GALACTOSE OXIDASE;
DACTYLLUM DENDROIDES MUTANT;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ;
0710 ;
09 ;
摘要：
Intracellular galactose oxidase (EC.1.1.3.9) was purified from a mutant (DD09) of the original enzyme-producing fungal strain Dactylium dendroides. This mutant strain has an impairment in the enzyme secretion. The enzyme is a protein of 70kDa containing 4.5% of neutral sugar residues of which 73% are resistent to beta - elimination. Unlike the wild type,the enzyme showed a higher affinity for dihydroxyacetone than for galactose.
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页码：907 / 912
页数：6
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