LYSOSOMAL INTEGRITY IN POSTMORTEM BOVINE SKELETAL-MUSCLE

被引:0
作者
CHAMBERS, JJ [1 ]
REVILLE, WJ [1 ]
ZEECE, MG [1 ]
机构
[1] NATL UNIV IRELAND UNIV COLL CORK,DEPT BIOCHEM,CORK,IRELAND
关键词
MUSCLE; AGING; LYSOSOME; MEMBRANE; ENZYME ACTIVITY;
D O I
暂无
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Post mortem aging of bovine skeletal muscle was found to result in loss of lysosomal integrity when assessed by biochemical and ultrastructural means. However, after aging to 14 days, the non-sedimentable beta-glucuronidase activity was only slightly higher than that at slaughter. Similarly, total non-sedimentable cathepsin D activity increased only slightly. Specific activity of cathepsin D increased, perhaps through proteolytic activation. Ultrastructural examination revealed that membrane integrity decreased substantially post mortem. These results suggest that a portion of the lysosome's enzyme content was released in response to normal aging and thus could participate in changes in meat texture post mortem.
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页码:441 / 457
页数:17
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